UniProt functional annotation for O31526



	UniProt functional annotation for O31526

UniProt code: O31526.

Organism: Bacillus subtilis (strain 168).

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.

Function: Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases oligosaccharide products (PubMed:16682770, PubMed:17449691, PubMed:19193638). Degrades rhamnogalacturonan, polygalacturonic acid, pectic acid and pectin (PubMed:16682770, PubMed:17449691). {ECO:0000269|PubMed:16682770, ECO:0000269|PubMed:17449691, ECO:0000269|PubMed:19193638}.

Catalytic activity: Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl- (1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5- unsaturated D-galactopyranosyluronic acid at the non-reducing end.; EC=4.2.2.23; Evidence={ECO:0000269|PubMed:17449691, ECO:0000269|PubMed:19193638};

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:16682770, ECO:0000269|PubMed:17449691, ECO:0000269|PubMed:17947240, ECO:0000269|PubMed:19193638}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:16682770, ECO:0000269|PubMed:17449691}; Note=Binds 10 calcium ions (PubMed:16682770, PubMed:17449691, PubMed:19193638). The calcium may have a structural role. Requires calcium or manganese for activity (PubMed:16682770, PubMed:17449691). {ECO:0000269|PubMed:16682770, ECO:0000269|PubMed:17449691, ECO:0000269|PubMed:19193638};

Biophysicochemical properties: Kinetic parameters: KM=0.181 mg/ml for rhamnogalacturonan (RG) type I region of plant cell wall pectin {ECO:0000269|PubMed:19193638}; pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:17449691}; Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269|PubMed:17449691};

Subunit: Monomer. {ECO:0000269|PubMed:16682770, ECO:0000269|PubMed:17947240, ECO:0000269|PubMed:19193638}.

Subcellular location: Secreted {ECO:0000269|PubMed:17449691}.

Induction: Up-regulated by growth on type I rhamnogalacturonan. {ECO:0000269|PubMed:17449691}.

Domain: Rhamnose is bound near the active site, but there are also additional rhamnose-binding sites far away from the active site, which possibly function as a carbohydrate-binding region. {ECO:0000269|PubMed:19193638}.

Similarity: Belongs to the polysaccharide lyase 11 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus subtilis (strain 168).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.



Function:		Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases oligosaccharide products (PubMed:16682770, PubMed:17449691, PubMed:19193638). Degrades rhamnogalacturonan, polygalacturonic acid, pectic acid and pectin (PubMed:16682770, PubMed:17449691). {ECO:0000269\|PubMed:16682770, ECO:0000269\|PubMed:17449691, ECO:0000269\|PubMed:19193638}.


Catalytic activity:		Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl- (1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5- unsaturated D-galactopyranosyluronic acid at the non-reducing end.; EC=4.2.2.23; Evidence={ECO:0000269\|PubMed:17449691, ECO:0000269\|PubMed:19193638};
Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269\|PubMed:16682770, ECO:0000269\|PubMed:17449691, ECO:0000269\|PubMed:17947240, ECO:0000269\|PubMed:19193638}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269\|PubMed:16682770, ECO:0000269\|PubMed:17449691}; Note=Binds 10 calcium ions (PubMed:16682770, PubMed:17449691, PubMed:19193638). The calcium may have a structural role. Requires calcium or manganese for activity (PubMed:16682770, PubMed:17449691). {ECO:0000269\|PubMed:16682770, ECO:0000269\|PubMed:17449691, ECO:0000269\|PubMed:19193638};
Biophysicochemical properties:		Kinetic parameters: KM=0.181 mg/ml for rhamnogalacturonan (RG) type I region of plant cell wall pectin {ECO:0000269\|PubMed:19193638}; pH dependence: Optimum pH is 8. {ECO:0000269\|PubMed:17449691}; Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269\|PubMed:17449691};
Subunit:		Monomer. {ECO:0000269\|PubMed:16682770, ECO:0000269\|PubMed:17947240, ECO:0000269\|PubMed:19193638}.
Subcellular location:		Secreted {ECO:0000269\|PubMed:17449691}.
Induction:		Up-regulated by growth on type I rhamnogalacturonan. {ECO:0000269\|PubMed:17449691}.
Domain:		Rhamnose is bound near the active site, but there are also additional rhamnose-binding sites far away from the active site, which possibly function as a carbohydrate-binding region. {ECO:0000269\|PubMed:19193638}.
Similarity:		Belongs to the polysaccharide lyase 11 family. {ECO:0000305}.