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UniProt functional annotation for Q47PU3 | ||
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| UniProt code: Q47PU3. |
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| Organism: | |
Thermobifida fusca (strain YX). |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae; Thermobifida. |
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| Function: | |
Catalyzes a Baeyer-Villiger oxidation reaction, i.e. the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Is most efficient with phenylacetone as substrate, leading to the formation of benzyl acetate. Can also oxidize other aromatic ketones (benzylacetone, alpha- methylphenylacetone and 4-hydroxyacetophenone), some aliphatic ketones (dodecan-2-one and bicyclohept-2-en-6-one) and sulfides (e.g. methyl 4- tolylsulfide). {ECO:0000269|PubMed:15599520}. |
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| Catalytic activity: | |
Reaction=H(+) + NADPH + O2 + phenylacetone = benzyl acetate + H2O + NADP(+); Xref=Rhea:RHEA:10124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:52051, ChEBI:CHEBI:52052, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.92; Evidence={ECO:0000269|PubMed:15599520}; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:15599520}; Note=Binds 1 FAD per subunit. {ECO:0000269|PubMed:15599520}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3 uM for NADPH {ECO:0000269|PubMed:15599520}; KM=59 uM for phenylacetone {ECO:0000269|PubMed:15599520}; KM=360 uM for benzylacetone {ECO:0000269|PubMed:15599520}; KM=830 uM for alpha-methylphenylacetone {ECO:0000269|PubMed:15599520}; KM=2.2 mM for 4-hydroxyacetophenone {ECO:0000269|PubMed:15599520}; KM=260 uM for 2-dodecanone {ECO:0000269|PubMed:15599520}; KM=15 mM for bicyclohept-2-en-6-one {ECO:0000269|PubMed:15599520}; KM=860 uM for methyl 4-tolylsulfide {ECO:0000269|PubMed:15599520}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:15599520}; Temperature dependence: Thermostable. Displays an activity half-life of 1 day at 52 degrees Celsius. {ECO:0000269|PubMed:15599520}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15328411, ECO:0000269|PubMed:15599520}. |
| Similarity: | |
Belongs to the FAD-binding monooxygenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.