PDBsum entry 2yls

Oxidoreductase

PDB id: 2yls

Contents

Protein chain

532 a.a.

Ligands

FAD

NAP

Waters ×238

PDB id:

2yls

Name:

Oxidoreductase

Title:

Snapshots of enzymatic baeyer-villiger catalysis: oxygen activation and intermediate stabilization: reduced enzyme bound to NADP

Structure:

Phenylacetone monooxygenase. Chain: a. Synonym: pamo, baeyer-villiger monooxygenase, bvmo. Engineered: yes

Source:

Thermobifida fusca. Organism_taxid: 2021. Expressed in: escherichia coli. Expression_system_taxid: 83333. Expression_system_variant: top10

Resolution:

2.26Å R-factor: 0.189 R-free: 0.235

Authors:

R.Orru,H.M.Dudek,C.Martinoli,D.E.Torres Pazmino,A.Royant,M.Weik, M.W.Fraaije,A.Mattevi

Key ref:

R.Orru et al. (2011). Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization. J Biol Chem, 286, 29284-29291. PubMed id: 21697090

Date:

06-Jun-11 Release date: 22-Jun-11

Protein chain

Q47PU3  (PAMO_THEFY) -  Phenylacetone monooxygenase from Thermobifida fusca (strain YX)

Seq: 542 a.a.

Struc: 532 a.a.

Enzyme reactions

• Enzyme class: E.C.1.14.13.92 - phenylacetone monooxygenase.
• Reaction: phenylacetone + NADPH + O2 + H⁺ = benzyl acetate + NADP⁺ + H2O

phenylacetone + NADPH (Bound ligand (Het Group name = NAP) corresponds exactly) + O2 + H(+) = benzyl acetate + NADP(+) + H2O

• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 286:29284-29291 (2011)

PubMed id: 21697090

Snapshots of enzymatic Baeyer-Villiger catalysis: oxygen activation and intermediate stabilization.

R.Orru, H.M.Dudek, C.Martinoli, D.E.Torres Pazmiño, A.Royant, M.Weik, M.W.Fraaije, A.Mattevi.

ABSTRACT

No abstract given.