|
|
||
|
|
|
|
|
UniProt functional annotation for Q89ZI2 | ||
|
|
|
|
|
|
||
| UniProt code: Q89ZI2. |
|
||
| Organism: | |
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482). |
| Taxonomy: | |
Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. |
|
||
|
||
| Function: | |
Can hydrolyze the glycosidic link of O-GlcNAcylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates (in vitro). {ECO:0000269|PubMed:16565725}. |
|
||
| Catalytic activity: | |
Reaction=3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = L-seryl-[protein] + N-acetyl-D-glucosamine; Xref=Rhea:RHEA:48876, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:12251, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:90838, ChEBI:CHEBI:506227; EC=3.2.1.169; Evidence={ECO:0000269|PubMed:16565725}; |
| Catalytic activity: | |
Reaction=3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine; Xref=Rhea:RHEA:48892, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:12252, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:90840, ChEBI:CHEBI:506227; EC=3.2.1.169; Evidence={ECO:0000269|PubMed:16565725}; |
| Activity regulation: | |
Inhibited by 1,2-dideoxy-2'-methyl-alpha-D- glucopyranoso-[2,1-d]-delta 2'-thiazoline (NAG-thiazoline) and O-(2- acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenyl-carbamate (PUGNAc). Not inhibited by Streptozotocin. {ECO:0000269|PubMed:16565725}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.30 mM for p-nitrophenyl-O-GlcNAc (pNP-O-GlcNAc) (at pH 7.4) {ECO:0000269|PubMed:16565725}; KM=0.28 mM for p-nitrophenyl-O-GlcNAc (pNP-O-GlcNAc) (at pH 6.5) {ECO:0000269|PubMed:16565725}; Vmax=3.2 umol/min/mg enzyme (at pH 7.4) {ECO:0000269|PubMed:16565725}; Vmax=19.9 umol/min/mg enzyme (at pH 6.5) {ECO:0000269|PubMed:16565725}; pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:16565725}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:16565725}. |
| Miscellaneous: | |
Metal-binding observed in X-ray crystal structures is artifactual. {ECO:0000305}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 84 family. {ECO:0000255}. |
Annotations taken from UniProtKB at the EBI.