PDBsum entry 2xii

Hydrolase

PDB id: 2xii

Contents

Protein chains

438 a.a. *

Ligands

TYR ×2

TA9 ×2

SO4 ×4

GOL

Waters ×696

* Residue conservation analysis

PDB id:

2xii

Name:

Hydrolase

Title:

Crystal structure of an alpha-l-fucosidase gh29 from bacteroides thetaiotaomicron in complex with an extended 9-fluorenone iminosugar inhibitor

Structure:

Alpha-l-fucosidase. Chain: a, b. Fragment: catalytic domain, 32-484. Synonym: fucosidase, glycoside hydrolase, alpha-l-fucoside fucohydrolase. Engineered: yes

Source:

Bacteroides thetaiotaomicron. Organism_taxid: 818. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.189 R-free: 0.223

Authors:

A.Lammerts Van Bueren,S.D.Popat,C.H.Lin,G.J.Davies

Key ref:

A.Lammerts van Bueren et al. (2010). Structural and thermodynamic analyses of α-L-fucosidase inhibitors. Chembiochem, 11, 1971-1974. PubMed id: 20665773

Date:

30-Jun-10 Release date: 21-Jul-10

Protein chains

Q8A3I4  (Q8A3I4_BACTN) -  Alpha-L-fucosidase from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)

Seq: 484 a.a.

Struc: 438 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.51 - alpha-L-fucosidase.
• Reaction: an alpha-L-fucoside + H2O = L-fucose + an alcohol

alpha-L-fucoside + H2O = L-fucose + alcohol (Bound ligand (Het Group name = TYR) matches with 50.00% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Chembiochem 11:1971-1974 (2010)

PubMed id: 20665773

Structural and thermodynamic analyses of α-L-fucosidase inhibitors.

A.Lammerts van Bueren, S.D.Popat, C.H.Lin, G.J.Davies.

ABSTRACT

No abstract given.