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PDBsum entry 2xg2
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Contents |
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84 a.a.
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94 a.a.
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71 a.a.
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60 a.a.
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56 a.a.
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59 a.a.
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45 a.a.
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49 a.a.
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64 a.a.
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36 a.a.
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191 a.a.
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272 a.a.
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205 a.a.
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208 a.a.
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181 a.a.
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165 a.a.
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145 a.a.
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131 a.a.
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139 a.a.
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122 a.a.
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146 a.a.
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141 a.a.
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117 a.a.
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99 a.a.
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138 a.a.
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117 a.a.
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101 a.a.
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113 a.a.
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93 a.a.
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101 a.a.
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187 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis for 16s ribosomal RNA cleavage by the cytotoxic domain of colicin e3.
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Authors
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C.L.Ng,
K.Lang,
N.A.Meenan,
A.Sharma,
A.C.Kelley,
C.Kleanthous,
V.Ramakrishnan.
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Ref.
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Nat Struct Biol, 2010,
17,
1241-1246.
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PubMed id
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Abstract
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No abstract given.
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Secondary reference #1
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Title
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Structure of the 70s ribosome complexed with mRNA and tRNA.
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Authors
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M.Selmer,
C.M.Dunham,
F.V.Murphy,
A.Weixlbaumer,
S.Petry,
A.C.Kelley,
J.R.Weir,
V.Ramakrishnan.
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Ref.
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Science, 2006,
313,
1935-1942.
[DOI no: ]
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PubMed id
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Figure 1.
Fig. 1. Representative electron density in the 70S structure.
(A) F[o] – F[c] map showing unbiased density of the acceptor
end of P-site tRNA (not included in the refinement), with
refined 3F[o] – 2F[c] density for 23S RNA. Base pairs between
C74 and C75 with G2252 and G2251 of 23S RNA are clearly visible.
(B) Simulated annealing omit map of the large subunit protein
L34 showing the visibility of well-ordered side chains. (C) A Mg
ion shown in unbiased difference Fourier maps that is
coordinated to the nonbridging phosphate oxygens at the 45°
mRNA kink between the A and P site as well as the nonbridging
phosphate oxygens of nucleotides 1400 and 1401 of h44 of 16S
RNA. All RNA is shown in final 3F[o] – 2F[c] density. The Mg
ions are shown in green. This and all other figures were made
with Pymol (47).
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Figure 5.
Fig. 5. E-site interactions in the 70S. (A) The anticodon of a
noncognate E-site tRNA does not interact with the E-site codon
of mRNA. Mg ions are shown in green. (B) A76 at the acceptor end
of E-site tRNA intercalates between bases G2421 and A2422 of 23S
RNA and interacts with the conserved base C2394. C75 is stacked
on C74 and is in a markedly different conformation from that
previously observed in an archaeal 50S subunit (38).
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The above figures are
reproduced from the cited reference
with permission from the AAAs
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