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PDBsum entry 2wk5
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References listed in PDB file
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Key reference
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Title
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Structures of native human thymidine phosphorylase and in complex with 5-Iodouracil.
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Authors
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E.Mitsiki,
A.C.Papageorgiou,
S.Iyer,
N.Thiyagarajan,
S.H.Prior,
D.Sleep,
C.Finnis,
K.R.Acharya.
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Ref.
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Biochem Biophys Res Commun, 2009,
386,
666-670.
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PubMed id
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Abstract
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Thymidine phosphorylase (TP) first identified as platelet derived endothelial
cell growth factor (PD-ECGF) plays a key role in nucleoside metabolism. Human TP
(hTP) is implicated in angiogenesis and is overexpressed in several solid
tumors. Here, we report the crystal structures of recombinant hTP and its
complex with a substrate 5-iodouracil (5IUR) at 3.0 and 2.5A, respectively. In
addition, we provide information on the role of specific residues in the
enzymatic activity of hTP through mutagenesis and kinetic studies.
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