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PDBsum entry 2waq
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Transcription
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PDB id
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2waq
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Contents |
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841 a.a.
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1090 a.a.
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367 a.a.
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260 a.a.
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174 a.a.
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90 a.a.
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113 a.a.
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74 a.a.
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82 a.a.
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91 a.a.
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64 a.a.
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43 a.a.
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45 a.a.
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References listed in PDB file
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Key reference
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Title
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Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
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Authors
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Y.Korkhin,
U.M.Unligil,
O.Littlefield,
P.J.Nelson,
D.I.Stuart,
P.B.Sigler,
S.D.Bell,
N.G.Abrescia.
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Ref.
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Plos Biol, 2009,
7,
e1000102.
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PubMed id
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Abstract
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The archaeal RNA polymerase (RNAP) shares structural similarities with
eukaryotic RNAP II but requires a reduced subset of general transcription
factors for promoter-dependent initiation. To deepen our knowledge of cellular
transcription, we have determined the structure of the 13-subunit DNA-directed
RNAP from Sulfolobus shibatae at 3.35 A resolution. The structure contains the
full complement of subunits, including RpoG/Rpb8 and the equivalent of the
clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have
identified subunit Rpo13, an RNAP component in the order Sulfolobales, which
contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and
RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation
of the transcription bubble.
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