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UniProt functional annotation for P10081 | ||
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| UniProt code: P10081. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. {ECO:0000269|PubMed:10409745, ECO:0000269|PubMed:10480875, ECO:0000269|PubMed:12535527, ECO:0000269|PubMed:1502180, ECO:0000269|PubMed:2169890, ECO:0000269|PubMed:2668952}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; Evidence={ECO:0000269|PubMed:12535527, ECO:0000269|PubMed:1502180}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=470 uM for ATP {ECO:0000269|PubMed:12535527, ECO:0000269|PubMed:1502180}; Vmax=2.6 pmol/sec/ug enzyme for ATP {ECO:0000269|PubMed:12535527, ECO:0000269|PubMed:1502180}; pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:12535527, ECO:0000269|PubMed:1502180}; |
| Subunit: | |
Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) (By similarity). Interacts with eIF4G1/TIF4631 and eIF4G2/TIF4632. {ECO:0000250, ECO:0000269|PubMed:10409745, ECO:0000269|PubMed:10480875}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:14562095}. |
| Domain: | |
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis. {ECO:0000269|PubMed:12535527}. |
| Miscellaneous: | |
TIF1 and TIF2 code for the same protein. |
| Miscellaneous: | |
Present with 106000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the DEAD box helicase family. eIF4A subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.