|
|
||
|
|
|
|
|
UniProt functional annotation for O69762 | ||
|
|
|
|
|
|
||
| UniProt code: O69762. |
|
||
| Organism: | |
Pseudomonas fluorescens. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
|
||
|
||
| Function: | |
Catalyzes the hydration of the acyl-CoA thioester of ferulic acid and the subsequent retro-aldol cleavage of the hydrated intermediate to yield vanillin (4-hydroxy-3-methoxy-benzaldehyde) (PubMed:9461612, PubMed:10222033). The enzyme is also active with caffeoyl-CoA and 4-coumaroyl-CoA producing 3,4-dihydroxybenzaldehyde and 4-hydroxybenaldehyde, respectively (PubMed:10222033). {ECO:0000269|PubMed:10222033, ECO:0000269|PubMed:9461612}. |
|
||
| Catalytic activity: | |
Reaction=(E)-caffeoyl-CoA + H2O = 3,4-dihydroxybenzaldehyde + acetyl- CoA; Xref=Rhea:RHEA:36307, ChEBI:CHEBI:15377, ChEBI:CHEBI:50205, ChEBI:CHEBI:57288, ChEBI:CHEBI:87136; EC=4.1.2.61; Evidence={ECO:0000269|PubMed:10222033}; |
| Catalytic activity: | |
Reaction=(E)-feruloyl-CoA + H2O = acetyl-CoA + vanillin; Xref=Rhea:RHEA:62412, ChEBI:CHEBI:15377, ChEBI:CHEBI:18346, ChEBI:CHEBI:57288, ChEBI:CHEBI:87305; EC=4.1.2.61; Evidence={ECO:0000269|PubMed:10222033, ECO:0000269|PubMed:9461612}; |
| Catalytic activity: | |
Reaction=(E)-4-coumaroyl-CoA + H2O = 4-hydroxybenzaldehyde + acetyl- CoA; Xref=Rhea:RHEA:62416, ChEBI:CHEBI:15377, ChEBI:CHEBI:17597, ChEBI:CHEBI:57288, ChEBI:CHEBI:85008; EC=4.1.2.61; Evidence={ECO:0000269|PubMed:10222033}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=11.8 uM for feruloyl-CoA {ECO:0000269|PubMed:18479250}; KM=2.4 uM for feruloyl-CoA {ECO:0000269|PubMed:10222033}; KM=5.3 uM for 4-coumaroyl-CoA {ECO:0000269|PubMed:10222033}; KM=1.6 uM for caffeoyl-CoA {ECO:0000269|PubMed:10222033}; Vmax=36.5 nmol/sec/mg enzyme with feruloyl-CoA as substrate {ECO:0000269|PubMed:10222033}; Vmax=73.3 nmol/sec/mg enzyme with 4-coumaroyl-CoA as substrate {ECO:0000269|PubMed:10222033}; Vmax=15.2 nmol/sec/mg enzyme with caffeoyl-CoA as substrate {ECO:0000269|PubMed:10222033}; Note=kcat is 3.72 sec(-1) with feruloyl-CoA as substrate. {ECO:0000269|PubMed:18479250}; pH dependence: Optimum pH is 8.5-9.5. {ECO:0000269|PubMed:10222033}; |
| Subunit: | |
Homohexamer; dimer of trimers. {ECO:0000269|PubMed:17139085, ECO:0000269|PubMed:18479250}. |
| Similarity: | |
Belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.