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PDBsum entry 2vq1
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Immune system
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PDB id
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2vq1
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References listed in PDB file
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Key reference
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Title
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Characterization of a diagnostic FAB fragment binding trimeric lewis X.
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Authors
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D.C.De geus,
A.M.Van roon,
E.A.Thomassen,
C.H.Hokke,
A.M.Deelder,
J.P.Abrahams.
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Ref.
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Proteins, 2009,
76,
439-447.
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PubMed id
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Abstract
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Lewis X trisaccharides normally function as essential cell-cell interaction
mediators. However, oligomers of Lewis X trisaccharides expressed by the
parasite Schistosoma mansoni seem to be related to its evasion of the immune
response of its human host. Here we show that monoclonal antibody 54-5C10-A,
which is used to diagnose schistosomiasis in humans, interacts with oligomers of
at least three Lewis X trisaccharides, but not with monomeric Lewis X. We
describe the sequence and the 2.5 A crystal structure of its Fab fragment and
infer a possible mode of binding of the polymeric Lewis X from docking studies.
Our studies indicate a radically different mode of binding compared to Fab
291-2G3-A, which is specific for monomeric Lewis X, thus providing a structural
explanation of the diagnostic success of 54-5C10-A.
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