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PDBsum entry 2vl2
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Oxidoreductase
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PDB id
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2vl2
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References listed in PDB file
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Key reference
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Title
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The crystal structures of oxidized forms of human peroxiredoxin 5 with an intramolecular disulfide bond confirm the proposed enzymatic mechanism for atypical 2-Cys peroxiredoxins.
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Authors
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A.Smeets,
C.Marchand,
D.Linard,
B.Knoops,
J.P.Declercq.
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Ref.
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Arch Biochem Biophys, 2008,
477,
98.
[DOI no: ]
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PubMed id
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Abstract
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Peroxiredoxin 5 (PRDX5) belongs to the PRDX superfamily of thiol-dependent
peroxidases able to reduce hydrogen peroxide, alkyl hydroperoxides and
peroxynitrite. PRDX5 is classified in the atypical 2-Cys subfamily of PRDXs. In
this subfamily, the oxidized form of the enzyme is characterized by the presence
of an intramolecular disulfide bridge between the peroxidatic and the resolving
cysteine residues. We report here three crystal forms in which this
intramolecular disulfide bond is indeed observed. The structures are
characterized by the expected local unfolding of the peroxidatic loop, but also
by the unfolding of the resolving loop. A new type of interface between PRDX
molecules is described. The three crystal forms were not oxidized in the same
way and the influence of the oxidizing conditions is discussed.
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