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PDBsum entry 2v0s
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Transcription
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PDB id
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2v0s
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References listed in PDB file
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Key reference
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Title
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Determinants for DNA target structure selectivity of the human line-1 retrotransposon endonuclease.
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Authors
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K.Repanas,
N.Zingler,
L.E.Layer,
G.G.Schumann,
A.Perrakis,
O.Weichenrieder.
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Ref.
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Nucleic Acids Res, 2007,
35,
4914-4926.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
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Abstract
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The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by
the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of
new L1 and Alu elements that presently account for approximately 28% of the
human genome. L1-EN bears considerable technological interest, because its
target selectivity may ultimately be engineered to allow the site-specific
integration of DNA into defined genomic locations. Based on the crystal
structure, we generated L1-EN mutants to analyze and manipulate DNA target site
recognition. Crystal structures and their dynamic and functional analysis show
entire loop grafts to be feasible, resulting in altered specificity, while
individual point mutations do not change the nicking pattern of L1-EN.
Structural parameters of the DNA target seem more important for recognition than
the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro
are less well defined than the respective integration site consensus in vivo.
This suggests that additional factors other than the DNA nicking specificity of
L1-EN contribute to the targeted integration of non-LTR retrotransposons.
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