 |
PDBsum entry 2uyk
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Unknown function
|
PDB id
|
|
|
|
2uyk
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
The crystal structure of escherichia coli tdcf, A member of the highly conserved yjgf/yer057c/uk114 family.
|
|
|
Authors
|
|
J.D.Burman,
C.E.Stevenson,
R.G.Sawers,
D.M.Lawson.
|
|
|
Ref.
|
|
Bmc Struct Biol, 2007,
7,
30.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
BACKGROUND: The YjgF/YER057c/UK114 family of proteins is widespread in nature,
but has as yet no clearly defined biological role. Members of the family exist
as homotrimers and are characterised by intersubunit clefts that are delineated
by well-conserved residues; these sites are likely to be of functional
significance, yet catalytic activity has never been detected for any member of
this family. The gene encoding the TdcF protein of E. coli, a YjgF/YER057c/UK114
family member, resides in an operon that strongly suggests a role in the
metabolism of 2-ketobutyrate for this protein. RESULTS: We have determined the
crystal structure of E. coli TdcF by molecular replacement to a maximum
resolution of 1.6 A. Structures are also presented of TdcF complexed with a
variety of ligands. CONCLUSION: The TdcF structure closely resembles those of
all YjgF/YER057c/UK114 family members determined thus far. It has the trimeric
quaternary structure and intersubunit cavities characteristic of this family of
proteins. We show that TdcF is capable of binding several low molecular weight
metabolites bearing a carboxylate group, although the interaction with
2-ketobutyrate appears to be the most well defined. These observations may be
indicative of a role for TdcF in sensing this potentially toxic metabolite.
|
|
|
|
|
|
|
