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UniProt functional annotation for Q9SCL7 | ||
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| UniProt code: Q9SCL7. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Involved in the arginine biosynthetic pathway via the intermediate compound ornithine. {ECO:0000269|PubMed:16377628, ECO:0000269|PubMed:16545809}. |
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| Catalytic activity: | |
Reaction=ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5- phosphate; Xref=Rhea:RHEA:14629, ChEBI:CHEBI:30616, ChEBI:CHEBI:44337, ChEBI:CHEBI:57936, ChEBI:CHEBI:456216; EC=2.7.2.8; |
| Activity regulation: | |
Inhibited by arginine. Inhibition is relieved by binding to GLB1. {ECO:0000269|PubMed:16377628, ECO:0000269|PubMed:19631611}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.08 mM for N-acetyl glutamate (NAG) {ECO:0000269|PubMed:16377628}; Vmax=10.6 mmol/min/mg enzyme with N-acetyl glutamate (NAG) as substrate {ECO:0000269|PubMed:16377628}; |
| Pathway: | |
Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl- L-ornithine from L-glutamate: step 2/4. |
| Subunit: | |
Interacts with GLB1. Interaction is dependent of MgATP and inhibited by 2-oxoglutarate, arginine, glutamate, citrate, and oxaloacetate. {ECO:0000269|PubMed:16377628, ECO:0000269|PubMed:17913711, ECO:0000269|PubMed:19631611}. |
| Subcellular location: | |
Plastid, chloroplast stroma {ECO:0000269|PubMed:16377628}. |
| Similarity: | |
Belongs to the acetylglutamate kinase family. ArgB subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.