UniProt functional annotation for Q9Y5X1



	UniProt functional annotation for Q9Y5X1

UniProt code: Q9Y5X1.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin- independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3- phosphate. {ECO:0000269|PubMed:11799118, ECO:0000269|PubMed:12952949, ECO:0000269|PubMed:15703209, ECO:0000269|PubMed:17609109, ECO:0000269|PubMed:17948057, ECO:0000269|PubMed:18388313, ECO:0000269|PubMed:20427313, ECO:0000269|PubMed:21048941, ECO:0000269|PubMed:22718350}.

Subunit: Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ACTR3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9. Interacts with FCHSD1 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails. {ECO:0000250|UniProtKB:Q91VH2, ECO:0000269|PubMed:10531379, ECO:0000269|PubMed:11799118, ECO:0000269|PubMed:12952949, ECO:0000269|PubMed:15703209, ECO:0000269|PubMed:16137687, ECO:0000269|PubMed:16316319, ECO:0000269|PubMed:17609109, ECO:0000269|PubMed:17948057, ECO:0000269|PubMed:18388313, ECO:0000269|PubMed:18940612, ECO:0000269|PubMed:19807924, ECO:0000269|PubMed:20129922, ECO:0000269|PubMed:20427313, ECO:0000269|PubMed:20491914}.

Subcellular location: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network. Cell projection, ruffle. Cytoplasm. Note=Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin- coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.

Tissue specificity: Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes. {ECO:0000269|PubMed:10531379}.

Domain: The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates. {ECO:0000269|PubMed:17948057}.

Ptm: Ubiquitinated by ITCH. {ECO:0000269|PubMed:20491914}.

Ptm: Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.

Similarity: Belongs to the sorting nexin family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin- independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3- phosphate. {ECO:0000269\|PubMed:11799118, ECO:0000269\|PubMed:12952949, ECO:0000269\|PubMed:15703209, ECO:0000269\|PubMed:17609109, ECO:0000269\|PubMed:17948057, ECO:0000269\|PubMed:18388313, ECO:0000269\|PubMed:20427313, ECO:0000269\|PubMed:21048941, ECO:0000269\|PubMed:22718350}.


Subunit:		Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ACTR3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9. Interacts with FCHSD1 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails. {ECO:0000250\|UniProtKB:Q91VH2, ECO:0000269\|PubMed:10531379, ECO:0000269\|PubMed:11799118, ECO:0000269\|PubMed:12952949, ECO:0000269\|PubMed:15703209, ECO:0000269\|PubMed:16137687, ECO:0000269\|PubMed:16316319, ECO:0000269\|PubMed:17609109, ECO:0000269\|PubMed:17948057, ECO:0000269\|PubMed:18388313, ECO:0000269\|PubMed:18940612, ECO:0000269\|PubMed:19807924, ECO:0000269\|PubMed:20129922, ECO:0000269\|PubMed:20427313, ECO:0000269\|PubMed:20491914}.
Subcellular location:		Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network. Cell projection, ruffle. Cytoplasm. Note=Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin- coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.
Tissue specificity:		Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes. {ECO:0000269\|PubMed:10531379}.
Domain:		The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates. {ECO:0000269\|PubMed:17948057}.
Ptm:		Ubiquitinated by ITCH. {ECO:0000269\|PubMed:20491914}.
Ptm:		Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.
Similarity:		Belongs to the sorting nexin family. {ECO:0000305}.