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UniProt functional annotation for Q9T0I8 | ||
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| UniProt code: Q9T0I8. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Enzyme of the methionine cycle that catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose (PubMed:19249293, PubMed:18342331). Contributes to the maintenance of AdoMet homeostasis and is required to sustain high rates of ethylene synthesis. Inactive towards S-adenosylhomocysteine (SAH/AdoHcy) (PubMed:18342331, PubMed:19249293). {ECO:0000269|PubMed:17144895, ECO:0000269|PubMed:18342331, ECO:0000269|PubMed:19249293, ECO:0000269|PubMed:20345605}. |
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| Catalytic activity: | |
Reaction=H2O + S-methyl-5'-thioadenosine = 5-(methylsulfanyl)-D-ribose + adenine; Xref=Rhea:RHEA:13617, ChEBI:CHEBI:15377, ChEBI:CHEBI:16708, ChEBI:CHEBI:17509, ChEBI:CHEBI:78440; EC=3.2.2.16; Evidence={ECO:0000269|PubMed:18342331, ECO:0000269|PubMed:19249293}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13618; Evidence={ECO:0000305|PubMed:18342331, ECO:0000305|PubMed:19249293}; |
| Activity regulation: | |
Inhibited by CBL3 in a calcium-dependent manner (PubMed:18945934). Inhibited by 5'-methylthiotubercidin (MTT) and by formycin A (FMA) (PubMed:18342331). {ECO:0000269|PubMed:18342331, ECO:0000269|PubMed:18945934}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.1 uM for 5'-methylthioadenosine (MTA) {ECO:0000269|PubMed:18342331}; KM=27.6 uM for 5'-methylthioadenosine (MTA) {ECO:0000269|PubMed:19249293}; Note=kcat is 18.7 sec(-1). {ECO:0000269|PubMed:18342331}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:18342331}; |
| Pathway: | |
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'- thioadenosine (hydrolase route): step 1/2. |
| Subunit: | |
Homodimer (PubMed:19249293, PubMed:16909418). Interacts with CBL3 in a calcium-dependent manner. {ECO:0000269|PubMed:16909418, ECO:0000269|PubMed:18342331, ECO:0000269|PubMed:18945934, ECO:0000269|PubMed:19249293, ECO:0000269|PubMed:20554051}. |
| Tissue specificity: | |
Expressed in roots, leaves, stems, cauline leaves and flowers. {ECO:0000269|PubMed:18945934}. |
| Disruption phenotype: | |
No visible phenotype under normal growth condition. Not able to grow with methylthioadenosine (MTA) as unique source of sulfur. {ECO:0000269|PubMed:20345605}. |
| Similarity: | |
Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.