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UniProt functional annotation for P9WP17 | ||
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| UniProt code: P9WP17. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate. It also acts as a dUTP diphosphatase. Affinity for dCTP and dUTP are very similar. {ECO:0000269|PubMed:18164314}. |
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| Catalytic activity: | |
Reaction=dCTP + 2 H2O = diphosphate + dUMP + NH4(+); Xref=Rhea:RHEA:19205, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:33019, ChEBI:CHEBI:61481, ChEBI:CHEBI:246422; EC=3.5.4.30; Evidence={ECO:0000255|HAMAP-Rule:MF_00146, ECO:0000269|PubMed:18164314}; |
| Activity regulation: | |
dTTP inhibits both the combined reaction and the dUTPase reaction. {ECO:0000269|PubMed:18164314}. |
| Biophysicochemical properties: | |
Kinetic parameters: Note=kcat is 0.18 sec(-1) with dCTP as substrate. kcat is 0.33 sec(- 1) with dUTP as substrate. {ECO:0000269|PubMed:18164314}; |
| Pathway: | |
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00146, ECO:0000305}. |
| Subunit: | |
Homotrimer. {ECO:0000255|HAMAP-Rule:MF_00146, ECO:0000269|PubMed:18164314}. |
| Similarity: | |
Belongs to the dCTP deaminase family. {ECO:0000255|HAMAP- Rule:MF_00146}. |
Annotations taken from UniProtKB at the EBI.