UniProt functional annotation for P60723



	UniProt functional annotation for P60723

UniProt code: P60723.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA (PubMed:3298242). It is important during the early stages of 50S assembly (PubMed:3298242). It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (PubMed:7556101, PubMed:6170935). {ECO:0000269|PubMed:13130133, ECO:0000269|PubMed:2442760, ECO:0000269|PubMed:3298242, ECO:0000269|PubMed:6170935, ECO:0000269|PubMed:7556101}.

Function: Protein L4 is a both a transcriptional repressor and a translational repressor protein; these two functions are independent of each other. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader; termination absolutely requires the NusA protein. L4 controls the translation of the S10 operon by binding to its mRNA. The regions of L4 that control regulation (residues 131-210) are different from those required for ribosome assembly (residues 89-103). {ECO:0000269|PubMed:13130133, ECO:0000269|PubMed:1692593, ECO:0000269|PubMed:2157208, ECO:0000269|PubMed:2442760}.

Function: Forms part of the polypeptide exit tunnel. {ECO:0000269|PubMed:11511371, ECO:0000269|PubMed:2442760}.

Function: Can regulate expression from Citrobacter freundii, Haemophilus influenzae, Morganella morganii, Salmonella typhimurium, Serratia marcescens, Vibrio cholerae and Yersinia enterocolitica (but not Pseudomonas aeruginosa) S10 leaders in vitro. {ECO:0000269|PubMed:10498727}.

Subunit: Part of the 50S ribosomal subunit (Ref.1, PubMed:7556101, PubMed:3298242, PubMed:10094780, PubMed:12809609, PubMed:11511371, PubMed:16272117, PubMed:25310980, PubMed:24844575, PubMed:27934701, PubMed:27906160, PubMed:27906161). {ECO:0000269|PubMed:10094780, ECO:0000269|PubMed:11511371, ECO:0000269|PubMed:12809609, ECO:0000269|PubMed:16272117, ECO:0000269|PubMed:24844575, ECO:0000269|PubMed:25310980, ECO:0000269|PubMed:27906160, ECO:0000269|PubMed:27906161, ECO:0000269|PubMed:27934701, ECO:0000269|PubMed:3298242, ECO:0000269|PubMed:7556101, ECO:0000269|Ref.1}.

Mass spectrometry: Mass=22086.2; Method=MALDI; Evidence={ECO:0000269|PubMed:10094780};

Miscellaneous: The erythromycin sensitive allele is dominant over the resistant allele.

Similarity: Belongs to the universal ribosomal protein uL4 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA (PubMed:3298242). It is important during the early stages of 50S assembly (PubMed:3298242). It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (PubMed:7556101, PubMed:6170935). {ECO:0000269\|PubMed:13130133, ECO:0000269\|PubMed:2442760, ECO:0000269\|PubMed:3298242, ECO:0000269\|PubMed:6170935, ECO:0000269\|PubMed:7556101}.



Function:		Protein L4 is a both a transcriptional repressor and a translational repressor protein; these two functions are independent of each other. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader; termination absolutely requires the NusA protein. L4 controls the translation of the S10 operon by binding to its mRNA. The regions of L4 that control regulation (residues 131-210) are different from those required for ribosome assembly (residues 89-103). {ECO:0000269\|PubMed:13130133, ECO:0000269\|PubMed:1692593, ECO:0000269\|PubMed:2157208, ECO:0000269\|PubMed:2442760}.



Function:		Forms part of the polypeptide exit tunnel. {ECO:0000269\|PubMed:11511371, ECO:0000269\|PubMed:2442760}.



Function:		Can regulate expression from Citrobacter freundii, Haemophilus influenzae, Morganella morganii, Salmonella typhimurium, Serratia marcescens, Vibrio cholerae and Yersinia enterocolitica (but not Pseudomonas aeruginosa) S10 leaders in vitro. {ECO:0000269\|PubMed:10498727}.


Subunit:		Part of the 50S ribosomal subunit (Ref.1, PubMed:7556101, PubMed:3298242, PubMed:10094780, PubMed:12809609, PubMed:11511371, PubMed:16272117, PubMed:25310980, PubMed:24844575, PubMed:27934701, PubMed:27906160, PubMed:27906161). {ECO:0000269\|PubMed:10094780, ECO:0000269\|PubMed:11511371, ECO:0000269\|PubMed:12809609, ECO:0000269\|PubMed:16272117, ECO:0000269\|PubMed:24844575, ECO:0000269\|PubMed:25310980, ECO:0000269\|PubMed:27906160, ECO:0000269\|PubMed:27906161, ECO:0000269\|PubMed:27934701, ECO:0000269\|PubMed:3298242, ECO:0000269\|PubMed:7556101, ECO:0000269\|Ref.1}.
Mass spectrometry:		Mass=22086.2; Method=MALDI; Evidence={ECO:0000269\|PubMed:10094780};
Miscellaneous:		The erythromycin sensitive allele is dominant over the resistant allele.
Similarity:		Belongs to the universal ribosomal protein uL4 family. {ECO:0000305}.