UniProt functional annotation for P0A7S3



	UniProt functional annotation for P0A7S3

UniProt codes: P0A7S3, P02367.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: With S4 and S5 plays an important role in translational accuracy.

Function: Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity). {ECO:0000250|UniProtKB:Q5SHN3, ECO:0000269|PubMed:7556101}.

Function: Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit. {ECO:0000305|PubMed:12093756}.

Subunit: Part of the 30S ribosomal subunit (PubMed:320034, PubMed:7556101, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:10094780, PubMed:12093756, PubMed:12379845, PubMed:12244297, PubMed:27906160, PubMed:27906161). Contacts proteins S8 and S17. Interacts with ArfA (PubMed:27906160, PubMed:27906161). May interact with IF1 in the 30S initiation complex (By similarity). {ECO:0000250|UniProtKB:Q5SHN3, ECO:0000269|PubMed:10094780, ECO:0000269|PubMed:12093756, ECO:0000269|PubMed:12244297, ECO:0000269|PubMed:12379845, ECO:0000269|PubMed:12809609, ECO:0000269|PubMed:16272117, ECO:0000269|PubMed:27906160, ECO:0000269|PubMed:27906161, ECO:0000269|PubMed:27934701, ECO:0000269|PubMed:320034, ECO:0000269|PubMed:7556101}.

Mass spectrometry: Mass=13651.3; Method=MALDI; Evidence={ECO:0000269|PubMed:10094780};

Miscellaneous: At least 19 substitutions or deletions in 11 codons can promote streptomycin resistance, dependence or pseudodependence; all but one of the streptomycin resistant mutations (K42R) are associated with hyperaccurate translation and thus reduced translational efficiency. {ECO:0000269|PubMed:1552908, ECO:0000269|Ref.5}.

Miscellaneous: The streptomycin sensitive allele is dominant to resistant alleles.

Similarity: Belongs to the universal ribosomal protein uS12 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		With S4 and S5 plays an important role in translational accuracy.



Function:		Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity). {ECO:0000250\|UniProtKB:Q5SHN3, ECO:0000269\|PubMed:7556101}.



Function:		Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit. {ECO:0000305\|PubMed:12093756}.


Subunit:		Part of the 30S ribosomal subunit (PubMed:320034, PubMed:7556101, PubMed:12809609, PubMed:16272117, PubMed:27934701, PubMed:10094780, PubMed:12093756, PubMed:12379845, PubMed:12244297, PubMed:27906160, PubMed:27906161). Contacts proteins S8 and S17. Interacts with ArfA (PubMed:27906160, PubMed:27906161). May interact with IF1 in the 30S initiation complex (By similarity). {ECO:0000250\|UniProtKB:Q5SHN3, ECO:0000269\|PubMed:10094780, ECO:0000269\|PubMed:12093756, ECO:0000269\|PubMed:12244297, ECO:0000269\|PubMed:12379845, ECO:0000269\|PubMed:12809609, ECO:0000269\|PubMed:16272117, ECO:0000269\|PubMed:27906160, ECO:0000269\|PubMed:27906161, ECO:0000269\|PubMed:27934701, ECO:0000269\|PubMed:320034, ECO:0000269\|PubMed:7556101}.
Mass spectrometry:		Mass=13651.3; Method=MALDI; Evidence={ECO:0000269\|PubMed:10094780};
Miscellaneous:		At least 19 substitutions or deletions in 11 codons can promote streptomycin resistance, dependence or pseudodependence; all but one of the streptomycin resistant mutations (K42R) are associated with hyperaccurate translation and thus reduced translational efficiency. {ECO:0000269\|PubMed:1552908, ECO:0000269\|Ref.5}.
Miscellaneous:		The streptomycin sensitive allele is dominant to resistant alleles.
Similarity:		Belongs to the universal ribosomal protein uS12 family. {ECO:0000305}.