|
|
||
|
|
|
|
|
UniProt functional annotation for Q9FK51 | ||
|
|
|
|
|
|
||
| UniProt code: Q9FK51. |
|
||
| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
|
||
|
||
| Function: | |
Catalyzes the conversion of ADP-glucose and inorganic phosphate (Pi) into glucose-1-phosphate and ADP. Does not possess galactose-1-phosphate uridylyltransferase activity. {ECO:0000269|PubMed:16519510}. |
|
||
| Catalytic activity: | |
Reaction=ADP + alpha-D-glucose 1-phosphate + H(+) = ADP-alpha-D-glucose + phosphate; Xref=Rhea:RHEA:47708, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:57498, ChEBI:CHEBI:58601, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:16519510}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:16519510, ECO:0000269|PubMed:17850744, ECO:0000269|Ref.6}; Note=Binds 2 zinc ions per subunit. {ECO:0000269|PubMed:16519510, ECO:0000269|PubMed:17850744, ECO:0000269|Ref.6}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=6.9 uM for ADP-alpha-D-glucose (at pH 8.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16519510}; KM=90 uM for phosphate (at pH 8.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16519510}; Note=kcat is 2.7 sec(-1). {ECO:0000269|PubMed:16519510}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:16519510, ECO:0000269|PubMed:17850744, ECO:0000269|Ref.6}. |
| Miscellaneous: | |
Functions by a double-displacement chemical mechanism and ping-pong kinetics through a covalent nucleotidyl-enzyme intermediate. {ECO:0000269|PubMed:16519510}. |
| Similarity: | |
Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.