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UniProt functional annotation for Q84MC2 | ||
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| UniProt code: Q84MC2. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms. {ECO:0000269|PubMed:19837870}. |
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| Catalytic activity: | |
Reaction=H2O + N(6)-(dimethylallyl)adenosine 5'-phosphate = D-ribose 5- phosphate + N(6)-dimethylallyladenine; Xref=Rhea:RHEA:48560, ChEBI:CHEBI:15377, ChEBI:CHEBI:17660, ChEBI:CHEBI:57526, ChEBI:CHEBI:78346; EC=3.2.2.n1; Evidence={ECO:0000269|PubMed:19837870}; |
| Catalytic activity: | |
Reaction=9-ribosyl-trans-zeatin 5'-phosphate + H2O = D-ribose 5- phosphate + trans-zeatin; Xref=Rhea:RHEA:48564, ChEBI:CHEBI:15377, ChEBI:CHEBI:16522, ChEBI:CHEBI:78346, ChEBI:CHEBI:87947; EC=3.2.2.n1; Evidence={ECO:0000269|PubMed:19837870}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=17 uM for N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate {ECO:0000269|PubMed:19837870}; Vmax=0.053 umol/min/mg enzyme with N(6)-(Delta(2)-isopentenyl)- adenosine 5'-phosphate as substrate {ECO:0000269|PubMed:19837870}; pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:19837870}; |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:19837870}. Nucleus {ECO:0000269|PubMed:19837870}. |
| Tissue specificity: | |
Expressed in roots and shoots. Detected in the root quiescent center and vasculature, in cotyledons, hypocotyls, stems, leaves, stomata, axillary buds, flowers and fruit abscission zones. {ECO:0000269|PubMed:19837870}. |
| Disruption phenotype: | |
No visible phenotype under normal growth conditions; due to the redundancy with other LOG proteins. {ECO:0000269|PubMed:19837870}. |
| Similarity: | |
Belongs to the LOG family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.