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PDBsum entry 2q0x
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Structural genomics, unknown function
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PDB id
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2q0x
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References listed in PDB file
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Key reference
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Title
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Structure of a trypanosoma brucei alpha/beta-Hydrolase fold protein with unknown function.
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Authors
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E.A.Merritt,
M.Holmes,
F.S.Buckner,
W.C.Van voorhis,
E.Quartly,
E.M.Phizicky,
A.Lauricella,
J.Luft,
G.Detitta,
H.Neely,
F.Zucker,
W.G.Hol.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2008,
64,
474-478.
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PubMed id
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Abstract
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The structure of a structural genomics target protein, Tbru020260AAA from
Trypanosoma brucei, has been determined to a resolution of 2.2 A using
multiple-wavelength anomalous diffraction at the Se K edge. This protein belongs
to Pfam sequence family PF08538 and is only distantly related to previously
studied members of the alpha/beta-hydrolase fold family. Structural
superposition onto representative alpha/beta-hydrolase fold proteins of known
function indicates that a possible catalytic nucleophile, Ser116 in the T.
brucei protein, lies at the expected location. However, the present structure
and by extension the other trypanosomatid members of this sequence family have
neither sequence nor structural similarity at the location of other active-site
residues typical for proteins with this fold. Together with the presence of an
additional domain between strands beta6 and beta7 that is conserved in
trypanosomatid genomes, this suggests that the function of these homologs has
diverged from other members of the fold family.
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