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UniProt functional annotation for P24197 | ||
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| UniProt code: P24197. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
In vitro, opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. The physiological substrate is unknown. {ECO:0000269|PubMed:23666480}. |
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| Catalytic activity: | |
Reaction=L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6- semialdehyde + H(+); Xref=Rhea:RHEA:21220, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57639; EC=1.13.11.29; Evidence={ECO:0000269|PubMed:23666480}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.9 mM for L-DOPA {ECO:0000269|PubMed:23666480}; Note=kcat is 0.17 min(-1) with L-DOPA. {ECO:0000269|PubMed:23666480}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:23666480}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:23666480}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:23666480}. |
| Miscellaneous: | |
Betalamic acid is the structural unit of the betalains, natural nitrogen-containing water-soluble pigments with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales. {ECO:0000305|PubMed:23666480}. |
| Similarity: | |
Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAC76075.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.