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PDBsum entry 2prf
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Actin-binding
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PDB id
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2prf
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References listed in PDB file
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Key reference
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Title
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Three-Dimensional solution structure of acanthamoeba profilin-I.
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Authors
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V.K.Vinson,
S.J.Archer,
E.E.Lattman,
T.D.Pollard,
D.A.Torchia.
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Ref.
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J Cell Biol, 1993,
122,
1277-1283.
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PubMed id
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Abstract
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We have determined a medium resolution three-dimensional solution structure of
Acanthamoeba profilin-I by multidimensional nuclear magnetic resonance
spectroscopy. This 13-kD actin binding protein consists of a five stranded
antiparallel beta sheet flanked by NH2- and COOH-terminal helices on one face
and by a third helix and a two stranded beta sheet on the other face. Data from
actin-profilin cross-linking experiments and the localization of conserved
residues between profilins in different phyla indicate that actin binding occurs
on the molecular face occupied by the terminal helices. The other face of the
molecule contains the residues that differ between Acanthamoeba profilins-I and
II and may be important in determining the difference in polyphosphoinositide
binding between these isoforms. This suggests that lipids and actin bind to
different faces of the molecule.
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Secondary reference #1
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Title
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Secondary structure and topology of acanthamoeba profilin i as determined by heteronuclear nuclear magnetic resonance spectroscopy.
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Authors
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S.J.Archer,
V.K.Vinson,
T.D.Pollard,
D.A.Torchia.
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Ref.
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Biochemistry, 1993,
32,
6680-6687.
[DOI no: ]
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PubMed id
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