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PDBsum entry 2plt
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Electron transport
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PDB id
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2plt
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References listed in PDB file
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Key reference
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Title
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The 1.5-A crystal structure of plastocyanin from the green alga chlamydomonas reinhardtii.
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Authors
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M.R.Redinbo,
D.Cascio,
M.K.Choukair,
D.Rice,
S.Merchant,
T.O.Yeates.
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Ref.
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Biochemistry, 1993,
32,
10560-10567.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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The crystal structure of plastocyanin from the green alga Chlamydomonas
reinhardtii has been determined at 1.5-A resolution with a crystallographic R
factor of 16.8%. Plastocyanin is a small (98 amino acids), blue copper-binding
protein that catalyzes the transfer of electrons in oxygenic photosynthesis from
cytochrome f in the quinol oxidase complex to P700+ in photosystem I.
Chlamydomonas reinhardtii plastocyanin is an eight-stranded, antiparallel
beta-barrel with a single copper atom coordinated in quasitetrahedral geometry
by two imidazole nitrogens (from His-37 and His-87), a cysteine sulfur (from
Cys-84), and a methionine sulfur (from Met-92). The molecule contains a region
of negative charge surrounding Tyr-83 (the putative distant site of electron
transfer) and an exclusively hydrophobic region surrounding His-87; these
regions are thought to be involved in the recognition of reaction partners for
the purpose of directing electron transfer. Chlamydomonas reinhardtii
plastocyanin is similar to the other plastocyanins of known structure,
particularly the green algal plastocyanins from Enteromorpha prolifera and
Scenedesmus obliquus. A potential "through-bond" path of electron transfer has
been identified in the protein that involves the side chain of Tyr-83, the
main-chain atoms between residues 83 and 84, the side chain of Cys-84, the
copper atom, and the side chain of His-87.
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Secondary reference #1
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Title
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Structure determination of plastocyanin from a specimen with a hemihedral twinning fraction of one-Half.
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Authors
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M.R.Redinbo,
T.O.Yeates.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1993,
49,
375-380.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Figure 1.
Fig. 1. The /3 = 70
° section of a cross-rotation function map
(Crowther, 1972) using
Enteromorpha
prolifera
plastocyanin as
a search model. Diffraction data between 15 and 2.5 A were
included and the Patterson radius cut off was 15 A. The map
was contoured at 2, 2.5, 3, 3.5, 4 and 4.51r. The peaks at y = 90
°
were the highest in the map. The periodicity of rr/3 in a illus-
trates the apparent sixfold symmetry about the z axis for the
observed data.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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