|
|
||
|
|
|
|
|
UniProt functional annotation for Q8F4I0 | ||
|
|
|
|
|
|
||
| UniProt code: Q8F4I0. |
|
||
| Organism: | |
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601). |
| Taxonomy: | |
Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira. |
|
||
|
||
| Function: | |
Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine (PubMed:17927957, PubMed:28581482). Utilizes a ping-pong kinetic mechanism in which the acetyl group of acetyl-CoA is initially transferred to the enzyme to form an acetyl- enzyme intermediate before subsequent transfer to homoserine to form the final product, O-acetylhomoserine (PubMed:17927957). {ECO:0000269|PubMed:17927957, ECO:0000269|PubMed:28581482}. |
|
||
| Catalytic activity: | |
Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine; Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31; Evidence={ECO:0000255|HAMAP-Rule:MF_00296, ECO:0000269|PubMed:17927957, ECO:0000269|PubMed:28581482}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.6 mM for L-homoserine {ECO:0000269|PubMed:17927957}; KM=0.95 mM for acetyl-CoA {ECO:0000269|PubMed:17927957}; |
| Pathway: | |
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00296}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00296, ECO:0000269|PubMed:17927957}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00296}. |
| Similarity: | |
Belongs to the AB hydrolase superfamily. MetX family. {ECO:0000255|HAMAP-Rule:MF_00296}. |
Annotations taken from UniProtKB at the EBI.