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PDBsum entry 2phf
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Sugar binding protein
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PDB id
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2phf
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References listed in PDB file
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Key reference
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Title
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How a plant lectin recognizes high mannose oligosaccharides.
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Authors
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A.Garcia-Pino,
L.Buts,
L.Wyns,
A.Imberty,
R.Loris.
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Ref.
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Plant Physiol, 2007,
144,
1733-1741.
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PubMed id
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Abstract
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The crystal structure of Pterocarpus angolensis seed lectin is presented in
complex with a series of high mannose (Man) oligosaccharides ranging from Man-5
to Man-9. Despite that several of the nine Man residues of Man-9 have the
potential to bind in the monosaccharide-binding site, all oligomannoses are
bound in the same unique way, employing the tetrasaccharide sequence
Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration
calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing
glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence
of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P.
angolensis seed lectin in a glycoprotein context.
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