PDBsum entry 2pha

Hydrolase

PDB id

2pha

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Contents

			Protein chain

					314 a.a. *

			Metals

					_MN ×4

			Waters ×254

* Residue conservation analysis

PDB id:

2pha

Links

Name:

Hydrolase

Title:

Crystal structure of native, unliganded human arginase at 1.90 resolution

Structure:

Arginase-1. Chain: a, b. Synonym: type i arginase, liver-type arginase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: arg1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å

R-factor:

0.198

R-free:

0.244

Authors:

L.Di Costanzo,M.E.Pique,D.W.Christianson

Key ref:

L.Di Costanzo et al. (2007). Crystal structure of human arginase I complexed with thiosemicarbazide reveals an unusual thiocarbonyl mu-sulfide ligand in the binuclear manganese cluster. J Am Chem Soc, 129, 6388-6389. PubMed id: 17469833

Date:

10-Apr-07

Release date:

08-May-07

PROCHECK

	Headers

	References

Protein chains

P05089 (ARGI1_HUMAN) - Arginase-1 from Homo sapiens

Seq: Struc:					322 a.a. 314 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.5.3.1 - arginase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Urea Cycle and Arginine Biosynthesis

Reaction:

L-arginine + H2O = urea + L-ornithine

L-arginine	+	H2O	=	urea	+	L-ornithine

Cofactor:

Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	J Am Chem Soc 129:6388-6389 (2007)
PubMed id: 17469833

Crystal structure of human arginase I complexed with thiosemicarbazide reveals an unusual thiocarbonyl mu-sulfide ligand in the binuclear manganese cluster.
L.Di Costanzo, M.E.Pique, D.W.Christianson.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20680216

C.Kozoni, E.Manolopoulou, M.Siczek, T.Lis, E.K.Brechin, and C.J.Milios (2010).
Polynuclear manganese amino acid complexes.

Dalton Trans, 39, 7943-7950.

20153713

L.Di Costanzo, M.Ilies, K.J.Thorn, and D.W.Christianson (2010).
Inhibition of human arginase I by substrate and product analogues.

Arch Biochem Biophys, 496, 101-108.

PDB codes:

3kv2 3lp4 3lp7

19093830

E.Y.Shishova, L.Di Costanzo, F.A.Emig, D.E.Ash, and D.W.Christianson (2009).
Probing the specificity determinants of amino acid recognition by arginase.

Biochemistry, 48, 121-131.

PDB codes:

3e6k 3e6v 3e8q 3e8z 3e9b

18360740

D.P.Dowling, L.Di Costanzo, H.A.Gennadios, and D.W.Christianson (2008).
Evolution of the arginase fold and functional diversity.

Cell Mol Life Sci, 65, 2039-2055.

18802628

T.Y.Zakharian, L.Di Costanzo, and D.W.Christianson (2008).
Synthesis of (2S)-2-amino-7,8-epoxyoctanoic acid and structure of its metal-bridging complex with human arginase I.

Org Biomol Chem, 6, 3240-3243.

PDB code:

3dj8

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.