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UniProt functional annotation for Q81IL5 | ||
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| UniProt code: Q81IL5. |
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| Organism: | |
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group. |
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| Function: | |
Catalyzes efficient racemization of N-succinyl-L-Arg and N- succinyl-L-Lys, suggesting that these are physiological substrates of this enzyme. Has low activity with L-Asp-L-Lys, and even lower activity with L-Leu-L-Arg, L-Leu-L-Lys, N-succinyl-L-His and N-succinyl-L-Met (in vitro). {ECO:0000269|PubMed:17603539}. |
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| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17603539}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:17603539}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.5 mM for N-succinyl-L-Arg {ECO:0000269|PubMed:17603539}; KM=3.0 mM for N-succinyl-L-Met {ECO:0000269|PubMed:17603539}; KM=11 mM for N-succinyl-L-His {ECO:0000269|PubMed:17603539}; KM=1.2 mM for L-Leu-L-Lys {ECO:0000269|PubMed:17603539}; KM=3.9 mM for L-Asp-L-Lys {ECO:0000269|PubMed:17603539}; KM=16 mM for L-Leu-L-Arg {ECO:0000269|PubMed:17603539}; |
| Miscellaneous: | |
Part of a large, functionally divergent protein family. Protein modeling and substrate docking was used to predict the substrate specificity, prior to biochemical analysis. |
| Similarity: | |
Belongs to the mandelate racemase/muconate lactonizing enzyme family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.