UniProt functional annotation for G5EEM5



	UniProt functional annotation for G5EEM5

UniProt code: G5EEM5.

Organism: Caenorhabditis elegans.

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis.

Function: Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos. Required for default nucleus positioning in oocytes. {ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:15866166, ECO:0000269|PubMed:16054029, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:2249759, ECO:0000269|PubMed:3949074, ECO:0000269|PubMed:6684994, ECO:0000269|PubMed:9606208}.

Subunit: Interacts with tac-1 to form a heterodimer. {ECO:0000269|PubMed:12956952}.

Subcellular location: Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:10085292, ECO:0000269|PubMed:12011109, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:9606208}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:10085292, ECO:0000269|PubMed:12011109, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:9606208}. Cytoplasm {ECO:0000269|PubMed:10085292, ECO:0000269|PubMed:12011109, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:9606208}. Note=Cycles between cytoplasm and centrosome during mitosis in a cell cycle- dependent manner. Centrosomal recruitment requires tbg-1.

Developmental stage: Expressed maternally during oogenesis. Observed during mitotic cell cycle from interphase through to early anaphase. {ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:3224814, ECO:0000269|PubMed:3949074, ECO:0000269|PubMed:9606208}.

Domain: The TOG (tumor overexpressed gene) domains are arranged in the N-terminal region with each domain composed of six (for the most part non-canonical) HEAT repeats and forming a oblong paddle-like structure. 3D-structure analysis shows the presence of additional HEAT repeats that are not detected by sequence-based prediction programs. Intra-HEAT loops are positioned along a face of the TOG domain and bind to a single alpha/beta-tubulin heterodimer. The TOG domains seem to be structurally and functionally polarized. Differential functions may range from microtubule (MT) lattice binding and/or free tubulin heterodimer binding to potentiating stable incorporation of tubulin into the MT lattice. {ECO:0000305, ECO:0000305|PubMed:17355870}.

Disruption phenotype: Maternal-effect embryonic lethal with meiotic and mitotic spindle positioning defects in one-cell stage embryos. {ECO:0000269|PubMed:12885567, ECO:0000269|PubMed:12956952, ECO:0000269|PubMed:15866166, ECO:0000269|PubMed:16054029, ECO:0000269|PubMed:1614224, ECO:0000269|PubMed:17666432, ECO:0000269|PubMed:2249759, ECO:0000269|PubMed:3949074, ECO:0000269|PubMed:6684994, ECO:0000269|PubMed:7371984, ECO:0000269|PubMed:9606208}.

Similarity: Belongs to the TOG/XMAP215 family. {ECO:0000255}.

Annotations taken from UniProtKB at the EBI.


Organism:		Caenorhabditis elegans.
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis.



Function:		Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos. Required for default nucleus positioning in oocytes. {ECO:0000269\|PubMed:12956952, ECO:0000269\|PubMed:15866166, ECO:0000269\|PubMed:16054029, ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:2249759, ECO:0000269\|PubMed:3949074, ECO:0000269\|PubMed:6684994, ECO:0000269\|PubMed:9606208}.


Subunit:		Interacts with tac-1 to form a heterodimer. {ECO:0000269\|PubMed:12956952}.
Subcellular location:		Cytoplasm, cytoskeleton, spindle pole {ECO:0000269\|PubMed:10085292, ECO:0000269\|PubMed:12011109, ECO:0000269\|PubMed:12956952, ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:9606208}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269\|PubMed:10085292, ECO:0000269\|PubMed:12011109, ECO:0000269\|PubMed:12956952, ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:9606208}. Cytoplasm {ECO:0000269\|PubMed:10085292, ECO:0000269\|PubMed:12011109, ECO:0000269\|PubMed:12956952, ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:9606208}. Note=Cycles between cytoplasm and centrosome during mitosis in a cell cycle- dependent manner. Centrosomal recruitment requires tbg-1.
Developmental stage:		Expressed maternally during oogenesis. Observed during mitotic cell cycle from interphase through to early anaphase. {ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:3224814, ECO:0000269\|PubMed:3949074, ECO:0000269\|PubMed:9606208}.
Domain:		The TOG (tumor overexpressed gene) domains are arranged in the N-terminal region with each domain composed of six (for the most part non-canonical) HEAT repeats and forming a oblong paddle-like structure. 3D-structure analysis shows the presence of additional HEAT repeats that are not detected by sequence-based prediction programs. Intra-HEAT loops are positioned along a face of the TOG domain and bind to a single alpha/beta-tubulin heterodimer. The TOG domains seem to be structurally and functionally polarized. Differential functions may range from microtubule (MT) lattice binding and/or free tubulin heterodimer binding to potentiating stable incorporation of tubulin into the MT lattice. {ECO:0000305, ECO:0000305\|PubMed:17355870}.
Disruption phenotype:		Maternal-effect embryonic lethal with meiotic and mitotic spindle positioning defects in one-cell stage embryos. {ECO:0000269\|PubMed:12885567, ECO:0000269\|PubMed:12956952, ECO:0000269\|PubMed:15866166, ECO:0000269\|PubMed:16054029, ECO:0000269\|PubMed:1614224, ECO:0000269\|PubMed:17666432, ECO:0000269\|PubMed:2249759, ECO:0000269\|PubMed:3949074, ECO:0000269\|PubMed:6684994, ECO:0000269\|PubMed:7371984, ECO:0000269\|PubMed:9606208}.
Similarity:		Belongs to the TOG/XMAP215 family. {ECO:0000255}.