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UniProt functional annotation for P53686 | ||
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| UniProt code: P53686. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
NAD-dependent histone deacetylase that is involved in nuclear silencing events. Derepresses subtelomeric silencing and increases repression in nucleolar (rDNA) silencing. Its function is negatively regulated by active nuclear export. {ECO:0000269|PubMed:10811920, ECO:0000269|PubMed:11106374, ECO:0000269|PubMed:11226170, ECO:0000269|PubMed:15274642, ECO:0000269|PubMed:17110954}. |
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| Catalytic activity: | |
Reaction=H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl- ADP-D-ribose + L-lysyl-[protein] + nicotinamide; Xref=Rhea:RHEA:43636, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:10731, ChEBI:CHEBI:15377, ChEBI:CHEBI:17154, ChEBI:CHEBI:29969, ChEBI:CHEBI:57540, ChEBI:CHEBI:61930, ChEBI:CHEBI:83767; EC=2.3.1.286; Evidence={ECO:0000255|PROSITE-ProRule:PRU00236, ECO:0000269|PubMed:16520376}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| Activity regulation: | |
Inhibited by ADP-ribose and nicotinamide. {ECO:0000269|PubMed:16520376}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=10.2 uM for NAD(+) {ECO:0000269|PubMed:11106374, ECO:0000269|PubMed:15274642, ECO:0000269|PubMed:16520376, ECO:0000269|PubMed:17289592}; KM=0.92 uM for acetylated poly-L-lysine {ECO:0000269|PubMed:11106374, ECO:0000269|PubMed:15274642, ECO:0000269|PubMed:16520376, ECO:0000269|PubMed:17289592}; KM=0.5 uM for a synthetic histone H3K14 acetyllysine peptide {ECO:0000269|PubMed:11106374, ECO:0000269|PubMed:15274642, ECO:0000269|PubMed:16520376, ECO:0000269|PubMed:17289592}; |
| Subunit: | |
Homotrimer. Monomer. Homotrimeric in its unliganded state. Undergoes a trimer-monomer transition upon acetyl-lysine substrate binding. {ECO:0000269|PubMed:14502267, ECO:0000269|PubMed:14604530, ECO:0000269|PubMed:15150415, ECO:0000269|PubMed:17289592}. |
| Subcellular location: | |
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and cytoplasm, but is largely cytoplasmic owing to efficient nuclear export. Nuclear exclusion is mediated by the exportin CRM1. |
| Miscellaneous: | |
Present with 5260 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the sirtuin family. Class I subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.