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UniProt functional annotation for Q9UR08 | ||
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| UniProt code: Q9UR08. |
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| Organism: | |
Aspergillus terreus. |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati. |
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| Function: | |
Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic sesquiterpene aristolochene. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce aristolochene. Aristolochene is the likely parent compound for a number of sesquiterpenoid toxins produced by filamentous fungi. {ECO:0000269|PubMed:10775423, ECO:0000269|PubMed:15186158}. |
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| Catalytic activity: | |
Reaction=(2E,6E)-farnesyl diphosphate = (+)-aristolochene + diphosphate; Xref=Rhea:RHEA:19825, ChEBI:CHEBI:33019, ChEBI:CHEBI:43445, ChEBI:CHEBI:175763; EC=4.2.3.9; Evidence={ECO:0000269|PubMed:10775423}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:18385128}; Note=Binds 3 Mg(2+) ions per monomer. {ECO:0000269|PubMed:18385128}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=13.5 nM for (2E,6E)-farnesyl diphosphate {ECO:0000269|PubMed:10775423, ECO:0000269|PubMed:15186158}; Vmax=23.6 nmol/min/mg enzyme {ECO:0000269|PubMed:10775423, ECO:0000269|PubMed:15186158}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:10775423, ECO:0000269|PubMed:15186158}; |
| Pathway: | |
Sesquiterpene biosynthesis; aristolochene biosynthesis; aristolochene from farnesyl diphosphate: step 1/1. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:17261032, ECO:0000269|PubMed:18385128}. |
| Similarity: | |
Belongs to the terpene synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.