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UniProt functional annotation for P33937 | ||
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| UniProt code: P33937. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite. {ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}. |
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| Catalytic activity: | |
Reaction=2 [Fe(II)cytochrome] + 2 H(+) + nitrate = 2 [Fe(III)cytochrome] + H2O + nitrite; Xref=Rhea:RHEA:12909, Rhea:RHEA- COMP:11777, Rhea:RHEA-COMP:11778, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034; EC=1.9.6.1; Evidence={ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}; |
| Cofactor: | |
Name=Mo-bis(molybdopterin guanine dinucleotide); Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}; Note=Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo- bis-MGD) cofactor per subunit. {ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:17130127}; |
| Activity regulation: | |
Interaction in the cytoplasm with the NapD chaperone prevents premature export. {ECO:0000269|PubMed:17901208}. |
| Subunit: | |
Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB (PubMed:10234835, PubMed:17130127). Before export to the periplasm, the NapA twin-arginine signal sequence interacts with NapD and NapF (PubMed:17074894, PubMed:17901208, PubMed:22329966, PubMed:24314029, Ref.13). {ECO:0000269|PubMed:10234835, ECO:0000269|PubMed:17074894, ECO:0000269|PubMed:17130127, ECO:0000269|PubMed:17901208, ECO:0000269|PubMed:22329966, ECO:0000269|PubMed:24314029, ECO:0000269|Ref.13}. |
| Subcellular location: | |
Periplasm {ECO:0000255|HAMAP-Rule:MF_01630, ECO:0000269|PubMed:10234835}. |
| Domain: | |
The twin-arginine signal peptide of NapA is structured in its unbound form and undergoes a small but significant conformational change upon interaction with NapD. {ECO:0000269|PubMed:24314029}. |
| Ptm: | |
Exported by the Tat system (PubMed:17218314). The position of the signal peptide cleavage has been experimentally proven (PubMed:10234835). {ECO:0000269|PubMed:10234835, ECO:0000269|PubMed:17218314}. |
| Similarity: | |
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. {ECO:0000255|HAMAP- Rule:MF_01630, ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA16399.1; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.