PDBsum entry 2nui

Hydrolase

PDB id: 2nui

Contents

Protein chain

124 a.a.

Ligands

BTB

Metals

_CL ×3

Waters ×155

References listed in PDB file

Key reference

• Title: Total synthesis by modern chemical ligation methods and high resolution (1.1 a) X-Ray structure of ribonuclease a.
• Authors: D.J.Boerema, V.A.Tereshko, S.B.Kent.
• Ref.: Biopolymers, 2008, 90, 278-286. [DOI no: 10.1002/bip.20800]
• PubMed id: 17610259

Abstract

The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.