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PDBsum entry 2n52
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Hydrolase inhibitor
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PDB id
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2n52
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References listed in PDB file
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Key reference
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Title
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The solution structure of the kallikrein-Related peptidases inhibitor spink6.
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Authors
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S.Jung,
J.Fischer,
B.Spudy,
T.Kerkow,
F.D.Sönnichsen,
L.Xue,
A.M.Bonvin,
P.Goettig,
V.Magdolen,
U.Meyer-Hoffert,
J.Grötzinger.
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Ref.
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Biochem Biophys Res Commun, 2016,
471,
103-108.
[DOI no: ]
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PubMed id
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Abstract
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Kallikrein-related peptidases (KLKs) are crucial for epidermal barrier function
and are involved in the proteolytic regulation of the desquamation process.
Elevated KLK levels were reported in atopic dermatitis. In skin, the proteolytic
activity of KLKs is regulated by specific inhibitors of the serine protease
inhibitor of Kazal-type (SPINK) family. SPINK6 was shown to be expressed in
human stratum corneum and is able to inhibit several KLKs such as KLK4, -5, -12,
-13 and -14. In order to understand the structural traits of the specific
inhibition we solved the structure of SPINK6 in solution by NMR-spectroscopy and
studied its interaction with KLKs. Thereby, beside the conserved binding mode,
we identified an alternate binding mode which has so far not been observed for
SPINK inhibitors.
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