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UniProt functional annotation for B3EWH0 | ||
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| UniProt code: B3EWH0. |
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| Organism: | |
Alopecosa marikovskyi (Wolf spider) (Lycosa kazakhstanicus). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae; Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Alopecosa. |
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| Function: | |
Enhances the high-affinity desensitization of human P2RX3 purinoceptors (PubMed:22842000). At 50 nM, the toxin decreases the IC(50) for ambient ATP from 2.67 nM to 0.77 nM in human P2RX3 (PubMed:22842000). {ECO:0000269|PubMed:22842000}. |
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| Subcellular location: | |
Secreted {ECO:0000269|PubMed:22842000}. |
| Tissue specificity: | |
Expressed by the venom gland. {ECO:0000305|PubMed:22842000}. |
| Domain: | |
The toxin is composed of 2 domains: a highly rigid N-terminal inhibitor cystine knot (knottin) domain and a rather flexible C- terminal linear cationic cytotoxin domain that forms amphiphilic alpha- helices. {ECO:0000269|PubMed:27412961}. |
| Domain: | |
The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin. {ECO:0000269|PubMed:27412961}. |
| Ptm: | |
Amidation at Leu-64 is not mandatory for activity on P2RX3. {ECO:0000269|PubMed:22842000}. |
| Mass spectrometry: | |
Mass=7255.5; Mass_error=0.5; Method=MALDI; Evidence={ECO:0000269|PubMed:22842000}; |
| Miscellaneous: | |
The toxin does not affect mouse P2RX2 or P2RX2/P2RX3 purinoceptors (PubMed:22842000). The toxin (and its derivatives composed of N-terminal or C-terminal domains) are inactive against a number of bacterial strains (PubMed:27412961). {ECO:0000269|PubMed:22842000, ECO:0000269|PubMed:27412961}. |
| Similarity: | |
Belongs to the neurotoxin 19 (CSTX) family. 05 (U4-Lctx) subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.