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UniProt functional annotation for P74313 | ||
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| UniProt code: P74313. |
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| Organism: | |
Synechocystis sp. (strain PCC 6803 / Kazusa). |
| Taxonomy: | |
Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis; unclassified Synechocystis. |
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| Function: | |
Reduces arsenate [As(V)] to arsenite [As(III)] using glutathione and glutaredoxin as sources of reducing equivalents. GrxA is the most effective electron donor in vivo compared to other glutaredoxins. Constitutes the major arsenate reductase compared to ArsI1 and ArsI2. Also shows weak phosphatase activity toward p- nitrophenyl phosphate. {ECO:0000269|PubMed:14617642, ECO:0000269|PubMed:19304854, ECO:0000269|PubMed:22155275}. |
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| Catalytic activity: | |
Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA- COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620; EC=3.1.3.48; |
| Catalytic activity: | |
Reaction=[glutaredoxin]-dithiol + arsenate + glutathione + 2 H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O; Xref=Rhea:RHEA:22016, Rhea:RHEA-COMP:10729, Rhea:RHEA-COMP:16283, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:29950, ChEBI:CHEBI:48597, ChEBI:CHEBI:57925, ChEBI:CHEBI:146199; EC=1.20.4.1; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=77 mM for p-nitrophenyl phosphate {ECO:0000269|PubMed:14617642}; Vmax=3.1 umol/min/mg enzyme with arsenate as substrate {ECO:0000269|PubMed:14617642}; Vmax=0.08 umol/min/mg enzyme with p-nitrophenyl phosphate as substrate {ECO:0000269|PubMed:14617642}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:14617642}. |
| Miscellaneous: | |
Cys-8 probably provides the initial functional group for covalent attachment of the electron-accepting substrate, arsenate, to ArsC. {ECO:0000305|PubMed:22155275}. |
| Similarity: | |
Belongs to the low molecular weight phosphotyrosine protein phosphatase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.