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PDBsum entry 2mw6
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References listed in PDB file
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Key reference
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Title
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Selective ruthenium labeling of the tryptophan residue in the bee venom peptide melittin.
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Authors
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D.S.Perekalin,
V.V.Novikov,
A.A.Pavlov,
I.A.Ivanov,
N.Y.Anisimova,
A.N.Kopylov,
D.S.Volkov,
I.F.Seregina,
M.A.Bolshov,
A.R.Kudinov.
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Ref.
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Chemistry, 2015,
21,
4923-4925.
[DOI no: ]
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PubMed id
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Abstract
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Melittin is a membrane-active peptide from bee venom with promising
antimicrobial and anticancer activity. Herein we report on a simple and
selective method for labeling of the tryptophan residue in melittin by the
organometallic fragment [(C5 H5 )Ru](+) in aqueous solution and in air.
Ruthenium coordination does not disturb the secondary structure of the peptide
(as verified by 2D NMR spectroscopy), but changes the pattern of its
intermolecular interactions resulting in an 11-fold decrease of hemolytic
activity. The high stability of the organometallic conjugate allowed the
establishment of the biodistribution of the labeled melittin in mice by
inductively coupled plasma MS analysis of ruthenium.
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