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PDBsum entry 2mrn
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DNA binding protein
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PDB id
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2mrn
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References listed in PDB file
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Key reference
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Title
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Escherichia coli antitoxin maze as transcription factor: insights into maze-Dna binding.
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Authors
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V.Zorzini,
L.Buts,
E.Schrank,
Y.G.Sterckx,
M.Respondek,
H.Engelberg-Kulka,
R.Loris,
K.Zangger,
N.A.Van nuland.
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Ref.
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Nucleic Acids Res, 2015,
43,
1241-1256.
[DOI no: ]
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PubMed id
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Abstract
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Toxin-antitoxin (TA) modules are pairs of genes essential for bacterial
regulation upon environmental stresses. The mazEF module encodes the MazF toxin
and its cognate MazE antitoxin. The highly dynamic MazE possesses an N-terminal
DNA binding domain through which it can negatively regulate its own promoter.
Despite being one of the first TA systems studied, transcriptional regulation of
Escherichia coli mazEF remains poorly understood. This paper presents the
solution structure of C-terminal truncated E. coli MazE and a MazE-DNA model
with a DNA palindrome sequence ∼10 bp upstream of the mazEF promoter. The work
has led to a transcription regulator-DNA model, which has remained elusive thus
far in the E. coli toxin-antitoxin family. Multiple complementary techniques
including NMR, SAXS and ITC show that the long intrinsically disordered
C-termini in MazE, required for MazF neutralization, does not affect the
interactions between the antitoxin and its operator. Rather, the MazE C-terminus
plays an important role in the MazF binding, which was found to increase the
MazE affinity for the palindromic single site operator.
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