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PDBsum entry 2mp2
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Protein binding
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PDB id
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2mp2
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References listed in PDB file
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Key reference
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Title
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Structural insight into sumo chain recognition and manipulation by the ubiquitin ligase rnf4.
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Authors
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Y.Xu,
A.Plechanovová,
P.Simpson,
J.Marchant,
O.Leidecker,
S.Kraatz,
R.T.Hay,
S.J.Matthews.
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Ref.
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Nat Commun, 2014,
5,
4217.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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The small ubiquitin-like modifier (SUMO) can form polymeric chains that are
important signals in cellular processes such as meiosis, genome maintenance and
stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO
chains on linked substrates and catalyses their ubiquitination, which targets
substrates for proteasomal degradation. Here we use a segmental labelling
approach combined with solution nuclear magnetic resonance (NMR) spectroscopy
and biochemical characterization to reveal how RNF4 manipulates the conformation
of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO
domain for ubiquitin transfer.
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