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PDBsum entry 2mkh
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Translation regulator
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PDB id
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2mkh
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References listed in PDB file
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Key reference
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Title
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A fly trap mechanism provides sequence-Specific RNA recognition by cpeb proteins.
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Authors
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T.Afroz,
L.Skrisovska,
E.Belloc,
J.Guillén-Boixet,
R.Méndez,
F.H.Allain.
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Ref.
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Genes Dev, 2014,
28,
1498-1514.
[DOI no: ]
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PubMed id
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Abstract
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Cytoplasmic changes in polyA tail length is a key mechanism of translational
control and is implicated in germline development, synaptic plasticity, cellular
proliferation, senescence, and cancer progression. The presence of a U-rich
cytoplasmic polyadenylation element (CPE) in the 3' untranslated regions (UTRs)
of the responding mRNAs gives them the selectivity to be regulated by the
CPE-binding (CPEB) family of proteins, which recognizes RNA via the tandem RNA
recognition motifs (RRMs). Here we report the solution structures of the tandem
RRMs of two human paralogs (CPEB1 and CPEB4) in their free and RNA-bound states.
The structures reveal an unprecedented arrangement of RRMs in the free state
that undergo an original closure motion upon RNA binding that ensures high
fidelity. Structural and functional characterization of the ZZ domain
(zinc-binding domain) of CPEB1 suggests a role in both protein-protein and
protein-RNA interactions. Together with functional studies, the structures
reveal how RNA binding by CPEB proteins leads to an optimal positioning of the
N-terminal and ZZ domains at the 3' UTR, which favors the nucleation of the
functional ribonucleoprotein complexes for translation regulation.
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