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PDBsum entry 2mi8
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Signaling protein
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PDB id
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2mi8
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References listed in PDB file
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Key reference
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Title
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Solution structure of lysine-Free (k0) ubiquitin.
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Authors
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T.Huang,
J.Li,
R.A.Byrd.
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Ref.
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Protein Sci, 2014,
23,
662-667.
[DOI no: ]
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PubMed id
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Abstract
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Lysine-free ubiquitin (K0-Ub) is commonly used to study the ubiquitin-signaling
pathway, where it is assumed to have the same structure and function as
wild-type ubiquitin (wt-Ub). However, the K0-Ub (15) N heteronuclear single
quantum correlation NMR spectrum differs significantly from wt-Ub and the
melting temperature is depressed by 19°C, raising the question of the
structural integrity and equivalence to wt-Ub. The three-dimensional structure
of K0-Ub was determined by solution NMR, using chemical shift and residual
dipolar coupling data. K0-Ub adopts the same backbone structure as wt-Ub, and
all significant chemical shifts can be related to interactions impacted by the K
to R mutations.
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