PDBsum entry 2mcf

Unknown function

PDB id: 2mcf

Contents

Protein chain

148 a.a.

PDB id:

2mcf

Name:

Unknown function

Title:

Nmr structure of tgam_1934

Structure:

Tgam_1934. Chain: a. Engineered: yes

Source:

Thermococcus gammatolerans. Organism_taxid: 593117. Strain: dsm 15229 / jcm 11827 / ej3. Gene: tgam_1934. Expressed in: escherichia coli. Expression_system_taxid: 562

NMR struc:

15 models

Authors:

Y.Yang,K.Montet De Guillen,C.Roumestand

Key ref:

Y.S.Yang et al. (2015). Prioritizing targets for structural biology through the lens of proteomics: the archaeal protein TGAM_1934 from Thermococcus gammatolerans. Proteomics, 15, 114-123. PubMed id: 25359407 DOI: 10.1002/pmic.201300535

Date:

19-Aug-13 Release date: 03-Sep-14

Protein chain

C5A217  (C5A217_THEGJ) -  Uncharacterized protein from Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)

Seq: 122 a.a.

Struc: 148 a.a.

DOI no: 10.1002/pmic.201300535

Proteomics 15:114-123 (2015)

PubMed id: 25359407

Prioritizing targets for structural biology through the lens of proteomics: the archaeal protein TGAM_1934 from Thermococcus gammatolerans.

Y.S.Yang, B.Fernandez, A.Lagorce, V.Aloin, K.M.De Guillen, J.B.Boyer, A.Dedieu, F.Confalonieri, J.Armengaud, C.Roumestand.

ABSTRACT

ORFans are hypothetical proteins lacking any significant sequence similarity with other proteins. Here, we highlighted by quantitative proteomics the TGAM_1934 ORFan from the hyperradioresistant Thermococcus gammatolerans archaeon as one of the most abundant hypothetical proteins. This protein has been selected as a priority target for structure determination on the basis of its abundance in three cellular conditions. Its solution structure has been determined using multidimensional heteronuclear NMR spectroscopy. TGAM_1934 displays an original fold, although sharing some similarities with the 3D structure of the bacterial ortholog of frataxin, CyaY, a protein conserved in bacteria and eukaryotes and involved in iron-sulfur cluster biogenesis. These results highlight the potential of structural proteomics in prioritizing ORFan targets for structure determination based on quantitative proteomics data. The proteomic data and structure coordinates have been deposited to the ProteomeXchange with identifier PXD000402 (http://proteomecentral.proteomexchange.org/dataset/PXD000402) and Protein Data Bank under the accession number 2mcf, respectively.