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PDBsum entry 2mcf
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Unknown function
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PDB id
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2mcf
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References listed in PDB file
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Key reference
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Title
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Prioritizing targets for structural biology through the lens of proteomics: the archaeal protein tgam_1934 from thermococcus gammatolerans.
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Authors
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Y.S.Yang,
B.Fernandez,
A.Lagorce,
V.Aloin,
K.M.De guillen,
J.B.Boyer,
A.Dedieu,
F.Confalonieri,
J.Armengaud,
C.Roumestand.
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Ref.
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Proteomics, 2015,
15,
114-123.
[DOI no: ]
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PubMed id
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Abstract
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ORFans are hypothetical proteins lacking any significant sequence similarity
with other proteins. Here, we highlighted by quantitative proteomics the
TGAM_1934 ORFan from the hyperradioresistant Thermococcus gammatolerans archaeon
as one of the most abundant hypothetical proteins. This protein has been
selected as a priority target for structure determination on the basis of its
abundance in three cellular conditions. Its solution structure has been
determined using multidimensional heteronuclear NMR spectroscopy. TGAM_1934
displays an original fold, although sharing some similarities with the 3D
structure of the bacterial ortholog of frataxin, CyaY, a protein conserved in
bacteria and eukaryotes and involved in iron-sulfur cluster biogenesis. These
results highlight the potential of structural proteomics in prioritizing ORFan
targets for structure determination based on quantitative proteomics data. The
proteomic data and structure coordinates have been deposited to the
ProteomeXchange with identifier PXD000402
(http://proteomecentral.proteomexchange.org/dataset/PXD000402) and Protein Data
Bank under the accession number 2mcf, respectively.
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