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PDBsum entry 2mc7
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Membrane protein
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PDB id
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2mc7
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References listed in PDB file
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Key reference
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Title
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Binding of mgtr, A salmonella transmembrane regulatory peptide, To mgtc, A mycobacterium tuberculosis virulence factor: a structural study.
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Authors
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F.L.Jean-Francois,
J.Dai,
L.Yu,
A.Myrick,
E.Rubin,
P.G.Fajer,
L.Song,
H.X.Zhou,
T.A.Cross.
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Ref.
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J Mol Biol, 2014,
426,
436-446.
[DOI no: ]
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PubMed id
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Abstract
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MgtR, a highly hydrophobic peptide expressed in Salmonella enterica serovar
Typhimurium, inhibits growth in macrophages through binding to the membrane
protein MgtC that has been identified as essential for replication in
macrophages. While the Mycobacterium tuberculosis MgtC is highly homologous to
its S. Typhi analogue, there does not appear to be an Mtb homologue for MgtR,
raising significant pharmacological interest in this system. Here, solid-state
NMR and EPR spectroscopy in lipid bilayer preparations were used to demonstrate
the formation of a heterodimer between S. Typhi MgtR and the transmembrane helix
4 of Mtb MgtC. Based on the experimental restraints, a structural model of this
heterodimer was developed using computational techniques. The result is that
MgtR appears to be ideally situated in the membrane to influence the
functionality of MgtC.
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