UniProt functional annotation for Q96NY9



	UniProt functional annotation for Q96NY9

UniProt code: Q96NY9.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. {ECO:0000269|PubMed:11741546, ECO:0000269|PubMed:12374758, ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:15805243, ECO:0000269|PubMed:17289582, ECO:0000269|PubMed:19595721, ECO:0000269|PubMed:19596235}.

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11741546};

Subunit: May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo. {ECO:0000269|PubMed:11741546, ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:15805243, ECO:0000269|PubMed:17289582, ECO:0000269|PubMed:18469862, ECO:0000269|PubMed:19595721, ECO:0000269|PubMed:19596235, ECO:0000269|PubMed:19596236}.

Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:11741546, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:14638871, ECO:0000269|PubMed:15805243}. Note=Recruited to foci of DNA damage in S-phase cells.

Tissue specificity: Widely expressed.

Developmental stage: Expressed in S phase and G2 phase. {ECO:0000269|PubMed:14638871}.

Induction: Up-regulated in cells treated with agents that damage DNA or block replication. This up-regulation seems to be independent of transcription. {ECO:0000269|PubMed:11741546}.

Similarity: Belongs to the XPF family. {ECO:0000305}.

Sequence caution: Sequence=BAB14953.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. {ECO:0000269\|PubMed:11741546, ECO:0000269\|PubMed:12374758, ECO:0000269\|PubMed:12686547, ECO:0000269\|PubMed:12721304, ECO:0000269\|PubMed:14617801, ECO:0000269\|PubMed:15805243, ECO:0000269\|PubMed:17289582, ECO:0000269\|PubMed:19595721, ECO:0000269\|PubMed:19596235}.


Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269\|PubMed:11741546};
Subunit:		May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo. {ECO:0000269\|PubMed:11741546, ECO:0000269\|PubMed:12686547, ECO:0000269\|PubMed:12721304, ECO:0000269\|PubMed:14617801, ECO:0000269\|PubMed:15805243, ECO:0000269\|PubMed:17289582, ECO:0000269\|PubMed:18469862, ECO:0000269\|PubMed:19595721, ECO:0000269\|PubMed:19596235, ECO:0000269\|PubMed:19596236}.
Subcellular location:		Nucleus, nucleolus {ECO:0000269\|PubMed:11741546, ECO:0000269\|PubMed:14617801, ECO:0000269\|PubMed:14638871, ECO:0000269\|PubMed:15805243}. Note=Recruited to foci of DNA damage in S-phase cells.
Tissue specificity:		Widely expressed.
Developmental stage:		Expressed in S phase and G2 phase. {ECO:0000269\|PubMed:14638871}.
Induction:		Up-regulated in cells treated with agents that damage DNA or block replication. This up-regulation seems to be independent of transcription. {ECO:0000269\|PubMed:11741546}.
Similarity:		Belongs to the XPF family. {ECO:0000305}.
Sequence caution:		Sequence=BAB14953.1; Type=Erroneous initiation; Evidence={ECO:0000305};