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PDBsum entry 2m1h
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Transcription
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PDB id
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2m1h
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References listed in PDB file
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Key reference
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Title
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Solution structure of tbtfiis2-1 pwwp domain from trypanosoma brucei.
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Authors
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R.Wang,
J.Zhang,
S.Liao,
X.Tu.
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Ref.
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Proteins, 2016,
84,
912-919.
[DOI no: ]
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PubMed id
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Abstract
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TbTFIIS2-1, one of the two TFIIS homologues of Trypanosome brucei (T. brucei),
cooperates with TbTFIIS1 in regulating transcription in T. brcuei. Structurally
divergent from other TFIIS homologues from higher organisms, TbTFIIS2-1 contains
an additional N-terminal PWWP domain besides other three conserved domains,
which may imply potential role of TbTFIIS2-1 in transcription regulation. Here,
we determined the solution structure of PWWP domain of TbTFIIS2-1 by NMR
spectroscopy, which was the first solution structure of PWWP domain solved in
trypanosomatid. In spite of poor sequence similarity between PWWP domains, this
domain of TbTFIIS2-1 adopts a conserved 3D-structure, which contains a
five-stranded β-barrel and a C-terminal α-helix. Furthermore, we found that
TbTFIIS2-1 PWWP domain may be a protein-protein interaction module without the
ability of DNA recognition and methyl-group interaction. Proteins 2016;
84:912-919. © 2016 Wiley Periodicals, Inc.
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