UniProt functional annotation for P9WIU5



	UniProt functional annotation for P9WIU5

UniProt code: P9WIU5.

Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).

Taxonomy: Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.

Function: Probably plays a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. The OmpA-like domain (196-326) binds M.tuberculosis peptidoglycan. Overexpression in M.bovis or M.smegmatis gives channels with average conductance value of 1,600 +/- 100 pS, but this may not be physiologically relevant. {ECO:0000269|PubMed:12366842, ECO:0000269|PubMed:17573469, ECO:0000269|PubMed:21410778}.

Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=May bind Zn(2+) via residues in the BON domain.;

Subunit: Controversial; may form oligomers (PubMed:17573469, PubMed:21117233), or not (PubMed:20199110, PubMed:21117233). {ECO:0000269|PubMed:17573469, ECO:0000269|PubMed:20199110, ECO:0000269|PubMed:21117233, ECO:0000269|PubMed:22108166}.

Subcellular location: Secreted, cell wall. Cell outer membrane. Note=Does not have a cleavable signal sequence.

Induction: Induced at low pH (at protein level), upon infecting a human monocytic cell line and in murine bone marrow macrophages. Part of the arfA-arfB-arfC operon. Maximal expression of ArfA requires the full operon. {ECO:0000269|PubMed:12366842, ECO:0000269|PubMed:21410778}.

Disruption phenotype: Significantly impaired growth at pH 5.5, reduced uptake of serine at both pH 7.2 and 5.5. Reduces growth in macrophages and in intravenously infected mice (PubMed:12366842). But the same mutant has very little effect when studied by another group (PubMed:21410778). Upon operon disruption no reduction of serine uptake at pH 6.9, no visible effect on outer membrane permeability, however severe delays in ammonia secretion, medium pH neutralization and growth also occur at pH 5.5 (PubMed:21410778). {ECO:0000269|PubMed:12366842, ECO:0000269|PubMed:21410778}.

Similarity: Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
Taxonomy:		Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.



Function:		Probably plays a role in ammonia secretion that neutralizes the medium at pH 5.5, although it does not play a direct role in ammonia transport. The OmpA-like domain (196-326) binds M.tuberculosis peptidoglycan. Overexpression in M.bovis or M.smegmatis gives channels with average conductance value of 1,600 +/- 100 pS, but this may not be physiologically relevant. {ECO:0000269\|PubMed:12366842, ECO:0000269\|PubMed:17573469, ECO:0000269\|PubMed:21410778}.


Cofactor:		Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=May bind Zn(2+) via residues in the BON domain.;
Subunit:		Controversial; may form oligomers (PubMed:17573469, PubMed:21117233), or not (PubMed:20199110, PubMed:21117233). {ECO:0000269\|PubMed:17573469, ECO:0000269\|PubMed:20199110, ECO:0000269\|PubMed:21117233, ECO:0000269\|PubMed:22108166}.
Subcellular location:		Secreted, cell wall. Cell outer membrane. Note=Does not have a cleavable signal sequence.
Induction:		Induced at low pH (at protein level), upon infecting a human monocytic cell line and in murine bone marrow macrophages. Part of the arfA-arfB-arfC operon. Maximal expression of ArfA requires the full operon. {ECO:0000269\|PubMed:12366842, ECO:0000269\|PubMed:21410778}.
Disruption phenotype:		Significantly impaired growth at pH 5.5, reduced uptake of serine at both pH 7.2 and 5.5. Reduces growth in macrophages and in intravenously infected mice (PubMed:12366842). But the same mutant has very little effect when studied by another group (PubMed:21410778). Upon operon disruption no reduction of serine uptake at pH 6.9, no visible effect on outer membrane permeability, however severe delays in ammonia secretion, medium pH neutralization and growth also occur at pH 5.5 (PubMed:21410778). {ECO:0000269\|PubMed:12366842, ECO:0000269\|PubMed:21410778}.
Similarity:		Belongs to the outer membrane OOP (TC 1.B.6) superfamily. ArfA family. {ECO:0000305}.