UniProt functional annotation for P08169



	UniProt functional annotation for P08169

UniProt code: P08169.

Organism: Bos taurus (Bovine).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.

Function: Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose- 6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4. {ECO:0000250|UniProtKB:P11717}.

Subunit: Binds HA-I and HA-II plasma membrane adapters (PubMed:2545438). Interacts with DPP4; the interaction is direct. Binds GGA1, GGA2 and GGA3 (By similarity). Interacts with the heterotrimeric retromer cargo-selective complex (CSC), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35; which is involved in retrograde trafficking of the receptor from endosomes to the Golgi apparatus (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P11717, ECO:0000269|PubMed:2545438}.

Subcellular location: Golgi apparatus membrane {ECO:0000250|UniProtKB:P11717}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P11717}. Endosome membrane {ECO:0000250|UniProtKB:P11717}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P11717}. Note=Mainly localized in the Golgi at steady state and not detectable in lysosome. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. {ECO:0000250|UniProtKB:P11717}.

Domain: Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.

Ptm: Palmitoylated. Undergoes cysteine S-palmitoylation which promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus. {ECO:0000250|UniProtKB:P11717}.

Similarity: Belongs to the MRL1/IGF2R family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bos taurus (Bovine).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.



Function:		Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose- 6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4. {ECO:0000250\|UniProtKB:P11717}.


Subunit:		Binds HA-I and HA-II plasma membrane adapters (PubMed:2545438). Interacts with DPP4; the interaction is direct. Binds GGA1, GGA2 and GGA3 (By similarity). Interacts with the heterotrimeric retromer cargo-selective complex (CSC), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35; which is involved in retrograde trafficking of the receptor from endosomes to the Golgi apparatus (By similarity). {ECO:0000250, ECO:0000250\|UniProtKB:P11717, ECO:0000269\|PubMed:2545438}.
Subcellular location:		Golgi apparatus membrane {ECO:0000250\|UniProtKB:P11717}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P11717}. Endosome membrane {ECO:0000250\|UniProtKB:P11717}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P11717}. Note=Mainly localized in the Golgi at steady state and not detectable in lysosome. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. {ECO:0000250\|UniProtKB:P11717}.
Domain:		Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
Ptm:		Palmitoylated. Undergoes cysteine S-palmitoylation which promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus. {ECO:0000250\|UniProtKB:P11717}.
Similarity:		Belongs to the MRL1/IGF2R family. {ECO:0000305}.